site-directed mutation meaning in Chinese

定点突变

The fusion protein was bactericidal active against staphylococcus aureus . in present study , we will truncate the none channel - forming do main , then attach the agrd to the pore - forming region ( k544 - i626 ) to construct a new engineered multidqmain protein machine - compact engineered peptide targeting staphylococcus aureus . such engineered peptide was constructed by linking the gene of staphylococcal agrd pheromone with the gene of c - terminal ( 1626 ) of colicin la pore - forming region ( k544 - i626 ) with site - directed mutation
利用点突变方法将金黄色葡萄球菌信息素agrd ( i型， ystcdfim )的基因引入到大肠菌素fa梭基端1626基因上，并将限制性内切酶sacl酶切位点基因分别引入到大肠菌素fa的p4和k544上，通过酶切、胶回收、连接获得含大肠菌素ia水性孔道结构域和金黄色葡萄球菌信息素agrd基因的重组质粒。